Sucralose artificial sweetener & Glycemic response

 1) Sucralose stands as most common non-nutrtive sweetner .

2,) Authors aim to study the effects of Sucralose daily intake on glycemiia

Glycemia means glucose in blood

Subjective appetite and gut microbiota.

Gut microbiota GM  useful microorganisms 

3)

changes in subjects with overweight or obesity. In this randomized, crossover, and controlled trial, 23 participants with a body mass index between 25 kg/m² and 39.9 kg/m² will be assigned to one of two interventions to receive either

 sucralose (2 mg/kg/day equivalent to 40% of the acceptable daily intake) or glucose (control) for 4 weeks, each phase separated by a 4-week washout period. The glycemic response will be determined during a meal tolerance test, subjective appetite will be evaluated using a visual analog scale, and GM changes will be analyzed by

( next-generation sequencing of the bacterial rRNA 16S gene from fecal samples). 

All measures will be performed before and after intervention periods. We hypothesize that sucralose supplementation induces changes in glycemic response, subjective appetite, and gut microbiota in overweight and obese participants. 

Introduction 

Global obesity and overweight rates have risen

 sharply, becoming a major health concern due to their association with type 2 diabetes (T2D) and cardiovascular disease. Mexico ranks second in obesity prevalence among the nations belonging to the Organization for Economic Cooperation and Development (OECD), with 36.9% of its population affected [1]. Non-nutritive sweeteners (NNS) have been implemented as substitutes for sugar to reduce caloric intake and prevent body weight gain..

Sucralose is an artificial NNS derived from sucrose, which has a sweetening capacity 600 times greater than sucrose and has an acceptable daily intake (ADI) estimated value of 5 mg/kg/day according to the Food and Drug Administration (FDA) [3].

 The use of sucralose is widely recommended among individuals with obesity and T2D.

 However, experimental and clinical trials have reported undesirable effects on glucose and energy metabolism,

 which can be attributed to three potential mechanisms: activation of T1R2/T1R3 sweet taste receptors, affecting insulin and incretin release; triggering a

 cephalic response that may increase caloric intake; and interaction with gut microbiota (GM), potentially causing dysbiosis. In the latter case, sucralose may disrupt GM composition,

 increasing intestinal permeability and allowing lipopolysaccharides (LPS) to enter the bloodstream, leading to a low-grade inflammatory response (metabolic endotoxemia) that could induce insulin resistance [4].

Ref

Methods Protoc. 2024 Oct 7;7(5):80. doi: 10.3390/mps7050080

Effects of Sucralose Supplementation on Glycemic Response, Appetite, and Gut Microbiota in Subjects with Overweight or Obesity: A Randomized Crossover Study Protocol

Zeniff Reyes-López 1, Viridiana Olvera-Hernández 1, Meztli Ramos-García 1, José D Méndez 2, Crystell G Guzmán-Priego 1, Miriam C Martínez-López 1, Carlos García-Vázquez 1, Carina S Alvarez-Villagomez 3, Isela E Juárez-Rojop 1, Juan C Díaz-Zagoya 4, Jorge L Ble-Castillo 1,*